常春藤茎段初代培养研究

利用正交设计表研究常春藤茎段诱导无菌苗的初代培养,试验结果经过方差分析及邓肯氏新复极差法显示:常春藤茎段腋芽萌发的最佳激素组合为:BA1.0mg/L+KT0.5mg/L+NAA0.1mg/L。     

橘皮黄色素的提取工艺优化及比较

采用浸渍法和索氏提取法提取柑橘皮中的黄色素,确定其最佳工艺条件。结果表明,浸渍法的最佳工艺条件是:以95%乙醇为提取剂,50℃浸渍3次,每次5h,共需15h;以相同的提取溶剂和稀释倍数,采用索氏提取法,在95℃水浴温度下,只需回流2h,就能优于浸渍法的提取效果。     

减少底肥施用量增加叶面施肥次数对玉米产量的影响

该试验目的是研究降低底肥施用量但是增加叶面肥施用次数方面对玉米产量的变化规律，旨在找出最经济的施肥方式以达到在保护环境的基础上提高经济效益的目的。试验表明，玉米产量随着底肥施用量的增加而提高，并且叶面肥喷施次数越多产量越高，每喷一次，可增加产量258~592.5 kg/hm2，增产幅度2.3%~5.6%。     

Effect of Alfalfa Flavonoids on Growth Performance and Serum Indexes of Sheep

This experiment was conducted to evaluate the effect of alfalfa flavonoids (Medicago sativa) on growth performance and serum indexes of sheep.This experiment was performed with hybrid F1 sheep of Suffolk sheep×Small-tailed Han sheep.Twenty-eight sheep with the body weight of (27.02±3.03) kg were randomly divided into 4 groups with 7 replicates in each group and 1 sheep per replicate.Sheep in different groups were fed diets with 0 (group Ⅰ), 0.1% (group Ⅱ), 0.2%(group Ⅲ) and 0.4% (group Ⅳ) alfalfa flavonoids, respectively.The experiment was cosisted of 7-day-preliminary trial and 41-day-formal trial.The results showed as follows:① Dietary supplementation of alfalfa flavonoids had no significant effects on growth performance of sheep(P>0.05).② Dietary supplementation of alfalfa flavonoids had significant effects on the contents of GLB, ASP, BUN, HDL and P in serum (P<0.05).Serum GLB content at the 1st day in group Ⅲ was significantly lower than that in groupsⅠand Ⅱ(P<0.05), serum ASP contents at the 1st and 21th days in group Ⅳ were significantly lower than those in group Ⅰ(P<0.05), serum BUN content at the 1st day in group Ⅳ was significantly lower than that in group Ⅰ(P<0.05), serum HDL content at the 21th day in group Ⅱ was significantly lower than that in groups Ⅰ and Ⅲ (P<0.05), serum P content in group Ⅳ at the 21th day was significantly lower than that in groups Ⅱ and Ⅲ (P<0.05).In conclusion, dietary supplementation of alfalfa flavonoids might modulate lipid metabolism, improve nitrogen utilization, maintain energy metabolic and balance Ca and P contents, and have the potential function for promoting growth and no adverse effect on liver.The results suggested that the appropriate supplemental level of alfalfa flavonoids for sheep was 0.2%.     

Liraglutide ameliorates liver injury of type 2 diabetic mice via micro-RNA-33-AMPK pathway

AIM: To observe the effects of liraglutide on the level of microRNA-33 (miR-33) and the expression of AMP-activated protein kinase (AMPK) and apoptosis-related proteins in mice with type 2 diabetes mellitus (T2DM), and to explore its possible mechanism. METHODS: High-fat diet and intraperitoneal injection of streptozocin were used to establish the type 2 diabetic model in C57BL/6 mice. The mice were randomly divided into 4 groups (n=15):in control group, the normal mice were subcutaneously injected with equivalent volume of saline; in model group, the T2DM mice were subcutaneously injected with equivalent volume of saline; in low-and high-dose liraglutide treatment groups, the T2DM mice were subcutaneously injected with 100 and 200 μg·kg^-1·d^-1, respectively. After 4 weeks of administration, the levels of FBG, TG, TC, HDL-C, LDL-C, ALT and AST were determined. HE staining was used to observe the pathological changes of the liver tissues. The protein level of cleaved caspase-3 in the liver tissue was detected by the technique of immunofluorescence. The protein levels of p-AMPK/AMPK and apoptosis-related proteins were detected by Western blot. The expression of miR-33 in the liver tissues was detected by real-time PCR. RESULTS: Compared with model group, the contents of FBG, TG, TC, LDL-C, ALT and AST were decreased significantly, while the content of HDL-C was increased significantly in low-dose liraglutide group and high-dose liraglutide group (P<0.05). The protein levels of phosphorylated AMPK and Bcl-2 were up-regulated significantly, and the expression of cleaved caspase-3 was down-regulated significantly (P<0.05). The level of miR-33 was decreased significantly (P<0.01). CONCLUSION: Liraglutide alleviates liver injury in type 2 diabetic mice, and the mechanism may be associated with reducing the level of miR-33 and increasing the phosphorylation of AMPK in the liver tissues, thereby inhibiting hepatocyte apoptosis.     

蛋鸡场清粪技术效能的层次分析法评价

蛋鸡场采用的清粪技术包括人工清粪、刮粪板清粪、传送带清粪等.本文通过现场调研不同地区不同规模的蛋鸡养殖场清粪技术,构建了层次分析法模型,从技术成熟度、技术应用效果、技术经济评价等方面对蛋鸡场清粪技术进行了效能评价.结果表明,蛋鸡场采用人工清粪、刮粪板清粪和传送带清粪技术效能得分分别为77.72、85.83和87.36,传送带清粪和刮粪板清粪技术的效能得分均显著高于人工清粪技术(P＜0.05),传送带清粪技术效能与刮粪板清粪技术间无显著差异(P＞0.05);随着蛋鸡存栏数的增加,传送带清粪技术的效能值增加,但不同规模未显著影响传送带清粪技术效能(P＞0.05);年存栏20万～50万只商品蛋鸡的蛋鸡场,采用刮粪板清粪、传送带清粪和人工清粪技术时效能值分别为85.83、84.00和77.72,3种清粪技术间差异不显著(P＞0.05).本研究可为不同规模和不同区域的蛋鸡场选用清粪技术时提供参考.     

动物生理学实验教学单独设课之重要性

在高校的动物生理学教学中,实验教学是其中的一个重要组成部分。作为课堂教学的重要辅助和补充,动物生理实验教学效果对课堂教学具有极大的驱动作用。为突出动物生理实验教学的重要性,对动物生理学实验施行单独设课,这是考查理论教学效果、促进实验课教学质量的一种重要措施。     

除虫菊地上部分水浸液对杂草种苗生长的影响

采用室内培养皿法,测试除虫菊地上部分水浸液对田间常见6种杂草种苗生长的影响。结果表明:不同质量浓度水浸液对杂草种苗生长的抑制作用依质量浓度梯度存在差异,除播娘蒿外其他5种总体均表现"低促高抑"趋势,在最高质量浓度0.03g·mL-1处理下,抑制作用最强;杂草种苗所受抑制作用大小依受体杂草种类存在差异,总体抑制作用由大到小依次为播娘蒿泽漆雀麦婆婆纳反枝苋野燕麦;除虫菊地上部分水浸液对试验杂草生理代谢影响测试表明,高质量浓度水浸液处理下,杂草幼苗受氧化胁迫严重。双子叶杂草较单子叶杂草对水浸液更敏感。     

梨树栽培管理关键技术

指出了梨树具有高度的营养价值、经济价值、生态价值,从林地的选择、林地整地、苗木栽植、树园管理、树形期修剪、果实采收贮运及梨树栽培管理技术等方面提出了关键技术实施要点,并对梨树的发展前景做出了评估.     

基于纳米碳添加的减量施肥模式对寒地水稻产量及氮肥效率的影响

为解决减量施肥造成北方寒地水稻肥料供给不足和产量低的问题,以纳米碳增效剂为材料,采用仪器快速检测和植株常规测定分析方法,研究纳米碳添加的常规施肥和减量施肥模式对水稻产量性状和肥料利用效率的影响。结果表明：与常规施肥(CK)相比,纳米碳添加的常规施肥模式(SM-A)、减施氮肥10%(SM-B)和减施氮肥20%(SM-C)对水稻植株形态特征、光合生理特性、产量性状和肥料利用效率产生显著的影响,其中以SM-B增幅最大,SM-A次之,SM-C最小;SM-A、SM-B和SM-C增加经济效益分别为563.56、1374.20和-1391.08元/hm ²,以SM-B增效值最高。本研究表明,SM-B能改善生育期光合生理特性,提高植株产量和肥料利用效率,是寒地水稻较为理想的减量施肥安全利用模式。